#THE NERVE BAND SERIES#
Due to the development of computer graphics, a series of two-dimensional images can be analyzed, recognized, and processed to establish a three-dimensional image. But this three-dimensional reconstruction is time-consuming and imprecise. Three-dimensional reconstruction in the early medical field generally uses a microscope projector or tracer to draw the contents of successive sections one by one, then engrave them on a wax plate or cardboard, and finally overlap them to form a three-dimensional model. Computer 3D reconstruction technology was not developed until the end of 1960s. On the basis of the microanatomical and histological studies of the internal structure of the common peroneal nerve, we attempted to reconstruct the three-dimensional image of the path of its functional tract. At present, there have been studies on the three-dimensional reconstruction of the internal functional bundle of the brachial plexus and the ulnar nerve, but there are few reports on the three-dimensional reconstruction of the internal functional bundle of the common peroneal nerve. Using computer technology to construct the three-dimensional image of the inner structure of the nerve can make up for the shortcomings mentioned above. Both the microanatomical map and histochemical staining sections were isolated sections, lacking continuity and relative positioning marks, which made it difficult to master the memory and could not meet the requirements of the randomized control adjustment of the binding site during the operation. However, due to the complexity of the internal structure of nerve, the type of nerve bundle changes greatly in a short distance, which makes these research results difficult to be applied in clinic. Through the study of the internal structure of the peripheral nerve, people have gained a deeper understanding of the changes in the type of the fiber bundles in the nerve as well as the qualitative and location of the fiber bundles, which has an important guiding significance for the accurate suture of the nerve bundles with the same functional properties. Based on histology and computer technology, the functional band of short peroneal nerve can be reconstructed in 3D, which provides a feasible basis for the three-dimensional reconstruction of the functional band of the long peripheral nerve. It has made preliminary achievements in brachial plexus, lumbosacral plexus, neural stem functional bundle (group), and intramuscular nerve routing and distribution, including the regeneration process of sensory nerve and three-dimensional reconstruction and visualization of composite tissue containing sensory nerve.
#THE NERVE BAND SOFTWARE#
Using Amira 3.1 three‐dimensional reconstruction software to realize the three-dimensional reconstruction and visualization of nerve can not only accurately perceive the complex three-dimensional surface structure of nerve, but also arbitrarily display, rotate, scale, and segment the three-dimensional structure inside nerve, and carry out three-dimensional measurement in time.
The properties of the functional bundles were manually judged. The stained sections were transformed into digital images by the digital camera system, and the images were stitched to obtain a two-dimensional panoramic image 100 times magnified. Acetylcholinesterase histochemical staining was used to observe the changes of nerve bundles under the microscope. The slices were 10 m thick, and the interval between the slices was 0.25 mm. A total of 200 sections of the 5cm-long popliteal fossa peroneal nerve from adult volunteers were cut and frozen. To explore the application of 3D image technology in motor and sensory nerve classification.
0 kommentar(er)
